Journal: Oncology reports

Article Title: Supervillin promotes tumor angiogenesis in liver cancer.

doi: 10.3892/or.2020.7621

Figure Lengend Snippet: Figure 1. SVIL is highly expressed in liver cancer and localized to new tumor vessels. (A) Analysis of microarray data from The Cancer Genome Atlas. (B) Immunohistochemistry was performed to detect the expression of SVIL in normal liver tissue, grade I, grade II and grade III liver cancer tissue samples (DAB staining; magnification, x100). (C) Liver cancer tissue was serially sectioned and analyzed for SVIL, CD31, CD34, CD146 and CD248 expression (DAB staining; magnification, x100). (D) CD34/PAS staining and SVIL/PAS staining were performed on the same liver cancer tissue area (DAB staining; magnification, x100 and x200). Statistics of the proportion of CD34‑labeled endothelial blood vessels in SVIL‑labeled tumor blood vessels. Data are presented as the mean ± standard deviation. SVIL, supervillin; TCGA, The Cancer Genome Atlas; PAS, periodic acid‑Schiff.

Article Snippet: A liver cancer tissue microarray consisting of 173 pathological samples was purchased from US Biomax, Inc. Liver cancer tissue microarrays were immunohistochemically treated with antibodies against SVIL (dilution 1:1,000; cat. no. S8695; Sigma-Aldrich, Inc.) (24), cluster of differentiation (CD) 31 (dilution 1:1,500; product no. 3528; Cell Signaling Technology, Inc.), CD34 (dilution 1:50; product no. 3569; Cell Signaling Technology, Inc.), CD146 (dilution 1:500; cat. no. GTX60775; GeneTex, Inc.) and CD248 (dilution 1:500; cat. no. 564993; BD Biosciences).

Techniques: Microarray, Immunohistochemistry, Expressing, Staining, Standard Deviation

Journal: Journal of cancer research and therapeutics

Article Title: Syk expression in non-small-cell lung cancer and its relation with angiogenesis.

doi: 10.4103/0973-1482.154082

Figure Lengend Snippet: Figure 2: CD34 immunostaining locates in microvascular endothelial cells with brown granular in tumor tissue SABC × 200

Article Snippet: Streptavidin‐biotin complex (SABC) kit, CD34 antibody, and DAB chromogenic reagent were from Wuhan Boster Biotechnology Company.

Techniques: Immunostaining

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Immunohistochemical staining images showing CD34 expression in ( A ) normal mouse brain tissue and ( B – F ) mouse brain tissue on days 3, 7, 15, 21 and 30 days, following the pTBI. ( A ) CD34 was not expressed in normal mouse brain tissue. CD34-positive cells and blood vessels (brown; black arrows) became visible at the injury site (red arrow) on days ( B ) 3 and ( C ) 7. ( D ) After 15 days, CD34 expression at the injury site (red arrow) decreased. ( E ) CD34 expression at the injury site could not be detected on day 21. Instead, black hemosiderin particles (black arrows) were observed at the injury site. ( F ) Some black hemosiderin particles remained visible at the injury site on day 30. ( G ) Immunohistochemical results of CD34 were analyzed by Image-Pro Plus 6.0 software. The average density (IOD) of CD34-positive cells in normal mouse brain tissue and in mouse brain tissue on days 3, 7, 15, 21 and 30 days, following pTBI. The n = 10/group was used for calculating the average IOD. Comparisons between groups were performed using the Kruskal–Wallis H test followed by Bonferroni post-hoc analysis.

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Software

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Double immunofluorescence staining images showing CD34/LYVE-1 expression in the brain tissue 3 days after pTBI. Expression of ( A ) LYVE-1 (green); ( B ) CD34 (red); and ( C ) merge (yellow).

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Double Immunofluorescence Staining, Expressing

Journal: Molecular Medicine Reports

Article Title: Method for in vitro differentiation of bone marrow mesenchymal stem cells into endothelial progenitor cells and vascular endothelial cells

doi: 10.3892/mmr.2016.5953

Figure Lengend Snippet: Formation of EPCs from human and canine bone marrow. Magnification: (A) ×200; (B) ×40; (C) ×200; (D) ×100; (E) ×40; (F) ×40; (G) ×100; (H) ×200. EPCs, endothelial progenitor cells.

Article Snippet: Sources of antibodies were as follows: Sheep anti-rabbit monoclonal rhodamine antibody (dilution 1:5,000, Promega, Madison, USA; cat no.: AS1270); FITC-labeled mouse monoclonal CD133 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 60577), mouse monoclonal CD31 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 3568); mouse monoclonal CD34 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 79253S); rabbit polyclonal VEGF antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab2349); and goat polyclonal factor VIII antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab139391); and CD34 magnetic separation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques:

Journal: Molecular Medicine Reports

Article Title: Method for in vitro differentiation of bone marrow mesenchymal stem cells into endothelial progenitor cells and vascular endothelial cells

doi: 10.3892/mmr.2016.5953

Figure Lengend Snippet: Flow cytometry detection on factor VIII-FITC-labeled endothelial progenitor cells (EPCs).

Article Snippet: Sources of antibodies were as follows: Sheep anti-rabbit monoclonal rhodamine antibody (dilution 1:5,000, Promega, Madison, USA; cat no.: AS1270); FITC-labeled mouse monoclonal CD133 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 60577), mouse monoclonal CD31 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 3568); mouse monoclonal CD34 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 79253S); rabbit polyclonal VEGF antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab2349); and goat polyclonal factor VIII antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab139391); and CD34 magnetic separation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Flow Cytometry, Labeling

Journal: Molecular Medicine Reports

Article Title: Method for in vitro differentiation of bone marrow mesenchymal stem cells into endothelial progenitor cells and vascular endothelial cells

doi: 10.3892/mmr.2016.5953

Figure Lengend Snippet: Immunofluorescence microscopic identification of the fifth generation cells. (A) Factor VIII-positive; (B) VEGF-positive; (C) CD133-positive; and (D) CD34-positive.

Article Snippet: Sources of antibodies were as follows: Sheep anti-rabbit monoclonal rhodamine antibody (dilution 1:5,000, Promega, Madison, USA; cat no.: AS1270); FITC-labeled mouse monoclonal CD133 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 60577), mouse monoclonal CD31 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 3568); mouse monoclonal CD34 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 79253S); rabbit polyclonal VEGF antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab2349); and goat polyclonal factor VIII antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab139391); and CD34 magnetic separation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Immunofluorescence

Journal: Molecular Medicine Reports

Article Title: Method for in vitro differentiation of bone marrow mesenchymal stem cells into endothelial progenitor cells and vascular endothelial cells

doi: 10.3892/mmr.2016.5953

Figure Lengend Snippet: In vitro induction and growth of human and canine bone marrow-derived EPCs. (A) Human EPCs. (B) Canine EPCs. EPCs, endothelial progenitor cells.

Article Snippet: Sources of antibodies were as follows: Sheep anti-rabbit monoclonal rhodamine antibody (dilution 1:5,000, Promega, Madison, USA; cat no.: AS1270); FITC-labeled mouse monoclonal CD133 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 60577), mouse monoclonal CD31 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 3568); mouse monoclonal CD34 antibody (1:50; Cell Signaling Technology, Danvers, MA, USA; cat no.: 79253S); rabbit polyclonal VEGF antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab2349); and goat polyclonal factor VIII antibody (dilution: 1:200, Abcam, Cambridge, MA, USA; cat no.: ab139391); and CD34 magnetic separation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: In Vitro, Derivative Assay